Isolation and characterisation of microsatellite loci for the ancient cephalopod, Nautilus pompilius

Rachel C. Williams; Deborah A. Dawson; Gavin J. Horsburgh; Billy Sinclair

doi:10.1007/s12686-014-0304-7

Isolation and characterisation of microsatellite loci for the ancient cephalopod, Nautilus pompilius

Rachel C. Williams
, Deborah A. Dawson
, Gavin J. Horsburgh
, Billy Sinclair

University of Cumbria

Research output: Contribution to journal › Journal Article › peer-review

Abstract

A microsatellite-enriched genomic library was created from a single Nautilus pompilius individual and clones/fragments sequenced using Sanger and Illumina MiSeq sequencing. Forty-four markers were tested in four individuals and 30 markers selected for further testing in a population of unrelated individuals from Imperieuse Reef, west Australia. Observed levels of heterozygosity in the IR population ranged from 0.17–1.00 with a mean of 17.2 alleles per locus. No groups of loci displayed evidence of significant linkage disequilibrium. Twenty-one markers were in Hardy–Weinberg equilibrium and will be used to study population structure in this species.

Original language	English
Pages (from-to)	107-111
Journal	Conservation Genetics Resources
Volume	7
Early online date	29 Aug 2014
DOIs	https://doi.org/10.1007/s12686-014-0304-7
Publication status	Published online - 29 Aug 2014

Keywords

cephalopod
conservation
microsatellite
mollusk
nautilus
shell trade

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10.1007/s12686-014-0304-7

http://dx.doi.org/10.1007/s12686-014-0304-7

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title = "Isolation and characterisation of microsatellite loci for the ancient cephalopod, Nautilus pompilius",

abstract = "A microsatellite-enriched genomic library was created from a single Nautilus pompilius individual and clones/fragments sequenced using Sanger and Illumina MiSeq sequencing. Forty-four markers were tested in four individuals and 30 markers selected for further testing in a population of unrelated individuals from Imperieuse Reef, west Australia. Observed levels of heterozygosity in the IR population ranged from 0.17–1.00 with a mean of 17.2 alleles per locus. No groups of loci displayed evidence of significant linkage disequilibrium. Twenty-one markers were in Hardy–Weinberg equilibrium and will be used to study population structure in this species.",

keywords = "cephalopod, conservation, microsatellite, mollusk, nautilus, shell trade",

author = "Williams, \{Rachel C.\} and Dawson, \{Deborah A.\} and Horsburgh, \{Gavin J.\} and Billy Sinclair",

year = "2014",

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day = "29",

doi = "10.1007/s12686-014-0304-7",

language = "English",

volume = "7",

pages = "107--111",

journal = "Conservation Genetics Resources",

issn = "1877-7252",

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TY - JOUR

T1 - Isolation and characterisation of microsatellite loci for the ancient cephalopod, Nautilus pompilius

AU - Williams, Rachel C.

AU - Dawson, Deborah A.

AU - Horsburgh, Gavin J.

AU - Sinclair, Billy

PY - 2014/8/29

Y1 - 2014/8/29

N2 - A microsatellite-enriched genomic library was created from a single Nautilus pompilius individual and clones/fragments sequenced using Sanger and Illumina MiSeq sequencing. Forty-four markers were tested in four individuals and 30 markers selected for further testing in a population of unrelated individuals from Imperieuse Reef, west Australia. Observed levels of heterozygosity in the IR population ranged from 0.17–1.00 with a mean of 17.2 alleles per locus. No groups of loci displayed evidence of significant linkage disequilibrium. Twenty-one markers were in Hardy–Weinberg equilibrium and will be used to study population structure in this species.

AB - A microsatellite-enriched genomic library was created from a single Nautilus pompilius individual and clones/fragments sequenced using Sanger and Illumina MiSeq sequencing. Forty-four markers were tested in four individuals and 30 markers selected for further testing in a population of unrelated individuals from Imperieuse Reef, west Australia. Observed levels of heterozygosity in the IR population ranged from 0.17–1.00 with a mean of 17.2 alleles per locus. No groups of loci displayed evidence of significant linkage disequilibrium. Twenty-one markers were in Hardy–Weinberg equilibrium and will be used to study population structure in this species.

KW - cephalopod

KW - conservation

KW - microsatellite

KW - mollusk

KW - nautilus

KW - shell trade

U2 - 10.1007/s12686-014-0304-7

DO - 10.1007/s12686-014-0304-7

M3 - Journal Article

SN - 1877-7252

VL - 7

SP - 107

EP - 111

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

ER -

Isolation and characterisation of microsatellite loci for the ancient cephalopod, Nautilus pompilius

Abstract

Keywords

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