Microbioreactor integrated with a sensor for monitoring intracellular green fluorescence protein (GFP)

Godfrey Pasirayi; Meez Islam; Simon M. Scott; Liam O'Hare; Zulfiqur Ali

doi:10.1007/978-3-319-00846-2_220

Microbioreactor integrated with a sensor for monitoring intracellular green fluorescence protein (GFP)

Godfrey Pasirayi
, Meez Islam
, Simon M. Scott
, Liam O'Hare
, Zulfiqur Ali

Senior Leadership Team

Teesside University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

A prototype Polydimethylsiloxane (PDMS) based microbioreactor integrated with a photodiode detector for monitoring intracellular green fluorescent protein ultraviolet (GFPUV) expression is described. The developed system is compact, simple and inexpensive thus making it ideal for an economical on chip detection of intracellular GFP and other biological applications. The detection limit for cell free GFPUV was found to be 4.8 ×10− 8 M while for Fluorescein isothiocyanate (FITC) was found to be 1.2 ×10− 6 M. The performance of the photodiode detector was benchmarked with a CCD spectrophotometer and results showed favorable comparison. This study demonstrates the quest to develop integrated microsystems that can be used to monitor cellular dynamics in real time.

Original language	English
Title of host publication	IFMBE Proceedings
Subtitle of host publication	XIII Mediterranean Conference on Medical and Biological Engineering and Computing 2013
Editors	Laura M. Roa Romero
Place of Publication	Cham, Switzerland
Publisher	Springer
Pages	888-891
Volume	41
ISBN (Electronic)	9783319008462
DOIs	https://doi.org/10.1007/978-3-319-00846-2_220
Publication status	Published - 2014
Event	Mediterranean Conference on Medical and Biological Engineering and Computing : Research and Development of Technology for Sustainable Healthcare - Seville, Spain Duration: 25 Sept 2013 → 28 Sept 2013 Conference number: XIII http://www.medicon2013.com/

Conference

Conference	Mediterranean Conference on Medical and Biological Engineering and Computing
Abbreviated title	MEDICON 2013
Country/Territory	Spain
City	Seville
Period	25/09/13 → 28/09/13
Internet address	http://www.medicon2013.com/

Access to Document

10.1007/978-3-319-00846-2_220

Cite this

Pasirayi, G., Islam, M., Scott, S. M., O'Hare, L., & Ali, Z. (2014). Microbioreactor integrated with a sensor for monitoring intracellular green fluorescence protein (GFP). In L. M. Roa Romero (Ed.), IFMBE Proceedings: XIII Mediterranean Conference on Medical and Biological Engineering and Computing 2013 (Vol. 41, pp. 888-891). Springer. https://doi.org/10.1007/978-3-319-00846-2_220

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title = "Microbioreactor integrated with a sensor for monitoring intracellular green fluorescence protein (GFP)",

abstract = "A prototype Polydimethylsiloxane (PDMS) based microbioreactor integrated with a photodiode detector for monitoring intracellular green fluorescent protein ultraviolet (GFPUV) expression is described. The developed system is compact, simple and inexpensive thus making it ideal for an economical on chip detection of intracellular GFP and other biological applications. The detection limit for cell free GFPUV was found to be 4.8 ×10− 8 M while for Fluorescein isothiocyanate (FITC) was found to be 1.2 ×10− 6 M. The performance of the photodiode detector was benchmarked with a CCD spectrophotometer and results showed favorable comparison. This study demonstrates the quest to develop integrated microsystems that can be used to monitor cellular dynamics in real time.",

author = "Godfrey Pasirayi and Meez Islam and Scott, \{Simon M.\} and Liam O'Hare and Zulfiqur Ali",

year = "2014",

doi = "10.1007/978-3-319-00846-2\_220",

language = "English",

volume = "41",

pages = "888--891",

editor = "\{Roa Romero\}, \{Laura M.\}",

booktitle = "IFMBE Proceedings",

publisher = "Springer",

note = "Mediterranean Conference on Medical and Biological Engineering and Computing<br/> : Research and Development of Technology for Sustainable Healthcare, MEDICON 2013 ; Conference date: 25-09-2013 Through 28-09-2013",

url = "http://www.medicon2013.com/",

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Pasirayi, G, Islam, M, Scott, SM, O'Hare, L & Ali, Z 2014, Microbioreactor integrated with a sensor for monitoring intracellular green fluorescence protein (GFP). in LM Roa Romero (ed.), IFMBE Proceedings: XIII Mediterranean Conference on Medical and Biological Engineering and Computing 2013. vol. 41, Springer, Cham, Switzerland, pp. 888-891, Mediterranean Conference on Medical and Biological Engineering and Computing
, Seville, Spain, 25/09/13. https://doi.org/10.1007/978-3-319-00846-2_220

Microbioreactor integrated with a sensor for monitoring intracellular green fluorescence protein (GFP). / Pasirayi, Godfrey; Islam, Meez; Scott, Simon M. et al.
IFMBE Proceedings: XIII Mediterranean Conference on Medical and Biological Engineering and Computing 2013. ed. / Laura M. Roa Romero. Vol. 41 Cham, Switzerland: Springer, 2014. p. 888-891.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Microbioreactor integrated with a sensor for monitoring intracellular green fluorescence protein (GFP)

AU - Pasirayi, Godfrey

AU - Islam, Meez

AU - Scott, Simon M.

AU - O'Hare, Liam

AU - Ali, Zulfiqur

N1 - Conference code: XIII

PY - 2014

Y1 - 2014

N2 - A prototype Polydimethylsiloxane (PDMS) based microbioreactor integrated with a photodiode detector for monitoring intracellular green fluorescent protein ultraviolet (GFPUV) expression is described. The developed system is compact, simple and inexpensive thus making it ideal for an economical on chip detection of intracellular GFP and other biological applications. The detection limit for cell free GFPUV was found to be 4.8 ×10− 8 M while for Fluorescein isothiocyanate (FITC) was found to be 1.2 ×10− 6 M. The performance of the photodiode detector was benchmarked with a CCD spectrophotometer and results showed favorable comparison. This study demonstrates the quest to develop integrated microsystems that can be used to monitor cellular dynamics in real time.

AB - A prototype Polydimethylsiloxane (PDMS) based microbioreactor integrated with a photodiode detector for monitoring intracellular green fluorescent protein ultraviolet (GFPUV) expression is described. The developed system is compact, simple and inexpensive thus making it ideal for an economical on chip detection of intracellular GFP and other biological applications. The detection limit for cell free GFPUV was found to be 4.8 ×10− 8 M while for Fluorescein isothiocyanate (FITC) was found to be 1.2 ×10− 6 M. The performance of the photodiode detector was benchmarked with a CCD spectrophotometer and results showed favorable comparison. This study demonstrates the quest to develop integrated microsystems that can be used to monitor cellular dynamics in real time.

U2 - 10.1007/978-3-319-00846-2_220

DO - 10.1007/978-3-319-00846-2_220

M3 - Conference contribution

VL - 41

SP - 888

EP - 891

BT - IFMBE Proceedings

A2 - Roa Romero, Laura M.

PB - Springer

CY - Cham, Switzerland

T2 - Mediterranean Conference on Medical and Biological Engineering and Computing<br/>

Y2 - 25 September 2013 through 28 September 2013

ER -

Pasirayi G, Islam M, Scott SM, O'Hare L, Ali Z. Microbioreactor integrated with a sensor for monitoring intracellular green fluorescence protein (GFP). In Roa Romero LM, editor, IFMBE Proceedings: XIII Mediterranean Conference on Medical and Biological Engineering and Computing 2013. Vol. 41. Cham, Switzerland: Springer. 2014. p. 888-891 doi: 10.1007/978-3-319-00846-2_220

Microbioreactor integrated with a sensor for monitoring intracellular green fluorescence protein (GFP)

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