Microbioreactor with photodiode detection for monitoring intracellular GFPUV expression in e. Coli

Godfrey Pasirayi; Meez Islam; Simon M. Scott; Liam O’Hare; Simon Bateson; Zulfiqur Ali

doi:10.2174/187640290602141127114324

Microbioreactor with photodiode detection for monitoring intracellular GFPUV expression in e. Coli

Godfrey Pasirayi
, Meez Islam
, Simon M. Scott
, Liam O’Hare
, Simon Bateson
, Zulfiqur Ali

Senior Leadership Team

Teesside University

Research output: Contribution to journal › Journal Article › peer-review

Abstract

A prototype membrane aerated polydimethylsiloxane (PDMS) microbioreactor integrated with a photodiode detector was developed for monitoring intracellular green fluorescent protein ultraviolet (GFPUV) expression in Escherichia coli. The developed system is compact, simple and inexpensive and ideal for economical on chip detection of fluorophores in aqueous solution and intracellular GFPUV expression. The detection limit for cell free GFPUV was found to be 4.8 x 10-8 M. Intracellular GFPUV expression in E. coli cells was monitored for 12 h and the lowest detectable signal was recorded from a cell concentration of 2.7 x 107cells/mL with signal to noise ratio (SNR) value of 3. The performance of the photodiode detector was benchmarked with a CCD spectrophotometer and results showed favorable comparison. A miniaturized microbioreactor for monitoring intracellular dynamics in real time is demonstrated.

Original language	English
Pages (from-to)	87-94
Number of pages	8
Journal	Micro and Nanosystems
Volume	6
Issue number	2
DOIs	https://doi.org/10.2174/187640290602141127114324
Publication status	Published - 2014

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title = "Microbioreactor with photodiode detection for monitoring intracellular GFPUV expression in e. Coli",

abstract = "A prototype membrane aerated polydimethylsiloxane (PDMS) microbioreactor integrated with a photodiode detector was developed for monitoring intracellular green fluorescent protein ultraviolet (GFPUV) expression in Escherichia coli. The developed system is compact, simple and inexpensive and ideal for economical on chip detection of fluorophores in aqueous solution and intracellular GFPUV expression. The detection limit for cell free GFPUV was found to be 4.8 x 10-8 M. Intracellular GFPUV expression in E. coli cells was monitored for 12 h and the lowest detectable signal was recorded from a cell concentration of 2.7 x 107cells/mL with signal to noise ratio (SNR) value of 3. The performance of the photodiode detector was benchmarked with a CCD spectrophotometer and results showed favorable comparison. A miniaturized microbioreactor for monitoring intracellular dynamics in real time is demonstrated.",

author = "Godfrey Pasirayi and Meez Islam and Scott, \{Simon M.\} and Liam O{\textquoteright}Hare and Simon Bateson and Zulfiqur Ali",

year = "2014",

doi = "10.2174/187640290602141127114324",

language = "English",

volume = "6",

pages = "87--94",

journal = "Micro and Nanosystems",

issn = "1876-4029",

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TY - JOUR

T1 - Microbioreactor with photodiode detection for monitoring intracellular GFPUV expression in e. Coli

AU - Pasirayi, Godfrey

AU - Islam, Meez

AU - Scott, Simon M.

AU - O’Hare, Liam

AU - Bateson, Simon

AU - Ali, Zulfiqur

PY - 2014

Y1 - 2014

N2 - A prototype membrane aerated polydimethylsiloxane (PDMS) microbioreactor integrated with a photodiode detector was developed for monitoring intracellular green fluorescent protein ultraviolet (GFPUV) expression in Escherichia coli. The developed system is compact, simple and inexpensive and ideal for economical on chip detection of fluorophores in aqueous solution and intracellular GFPUV expression. The detection limit for cell free GFPUV was found to be 4.8 x 10-8 M. Intracellular GFPUV expression in E. coli cells was monitored for 12 h and the lowest detectable signal was recorded from a cell concentration of 2.7 x 107cells/mL with signal to noise ratio (SNR) value of 3. The performance of the photodiode detector was benchmarked with a CCD spectrophotometer and results showed favorable comparison. A miniaturized microbioreactor for monitoring intracellular dynamics in real time is demonstrated.

AB - A prototype membrane aerated polydimethylsiloxane (PDMS) microbioreactor integrated with a photodiode detector was developed for monitoring intracellular green fluorescent protein ultraviolet (GFPUV) expression in Escherichia coli. The developed system is compact, simple and inexpensive and ideal for economical on chip detection of fluorophores in aqueous solution and intracellular GFPUV expression. The detection limit for cell free GFPUV was found to be 4.8 x 10-8 M. Intracellular GFPUV expression in E. coli cells was monitored for 12 h and the lowest detectable signal was recorded from a cell concentration of 2.7 x 107cells/mL with signal to noise ratio (SNR) value of 3. The performance of the photodiode detector was benchmarked with a CCD spectrophotometer and results showed favorable comparison. A miniaturized microbioreactor for monitoring intracellular dynamics in real time is demonstrated.

U2 - 10.2174/187640290602141127114324

DO - 10.2174/187640290602141127114324

M3 - Journal Article

SN - 1876-4029

VL - 6

SP - 87

EP - 94

JO - Micro and Nanosystems

JF - Micro and Nanosystems

IS - 2

ER -

Microbioreactor with photodiode detection for monitoring intracellular GFPUV expression in e. Coli

Abstract

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